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KMID : 0613820060160050866
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Journal of Life Science
2006 Volume.16 No. 5 p.866 ~ p.869
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Inhibitory Effect of Pine Needle( Pinus densiflora S.) Extract on Potato Polyphenol Oxidase
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Lee Min-Kyung
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Abstract
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The inhibitory effect of pine needle (Pinus densiflora S.) on potato polyphenol oxidase (PPO) was investigated. The addition of the pine needle extract exhibited a higher inhibitory effect on the potato polyphenol oxidase activity than that of the citric acid or potassium sorbate. The enzyme activity was strongly inhibited in a pH range of 7.0-8.0. When the incubation time of reaction mixture was increased, the potato polyphenol oxidase activity was markedly inhibited. The pine needle extract inhibited the potato polyphenol oxidase non-competitively. And also the pine needle extract subjected to a heat treatment at 100℃ for 10 min or to an acid treatment at pH 2.0, 3.0, and 4.0 for 3 hours still retained inhibitory effect on potato polyphenol oxidase.
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KEYWORD
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Pine needle extract, polyphenol oxidase, potato
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